2021-07-05 Tris hydroxy aminomethane production method First dissolve Tris with a small amount of double-distilled water (300~500ml), add HCl, adjust the pH to 7.6 with HCl (1N) or NaOH (1N), and finally add double-distilled water to 1000ml. This solution is a stock solution, stored in a refrigerator at 4°C. N