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First dissolve Tris with a small amount of double-distilled water (300~500ml), add HCl, adjust the pH to 7.6 with HCl (1N) or NaOH (1N), and finally add double-distilled water to 1000ml. This solution is a stock solution, stored in a refrigerator at 4°C. Note: The PH value of Tris-HCl changes with temperature, so you should wait until room temperature to test again, so that the measured results are more reliable.
Tris hydroxy aminomethane uses Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris is used for protein crystal growth under different pH conditions.
The low ionic strength of Tris buffer can be used to form the intermediate fibers of C. elegans lamin.  Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Tris is a weak base. At room temperature (25°C), its pKa is 8.1; according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH of the aqueous solution of Tris base is about 10.5. Generally, hydrochloric acid is added to adjust the pH to the desired value to obtain a buffer of the pH value. But at the same time, attention should be paid to the influence of temperature on the pKa of Tris.